强光照和黑暗条件下刺参体壁的基因表达差异     

张立斌  冯其明  孙丽娜  房燕  徐冬雪  张涛  杨红生 《海洋学报(英文版)》2018,37(5):54-66

参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。  相似文献   

CORROSION RESISTANCE OF INTERFERENCE FILM ON STAINLESS STEEL     

张经磊  侯保荣 《中国海洋湖沼学报》1997,15(4):363-368

IwrRODUcrlONStainlessstalhasbeenusedwidelyasbuildingrnateria1,especiallyasdecoratingrnate-rialinbuildings.Ordinarystainlesssteehasonlyonemonot0noussilverywhitCcolourwhichsomeimesdoesnotrnatchwithitSsurmundingenvironment.lnordert0improveitSdereratingperfonnaneeandexpanditsuse,muchresearchhasbeenconductedsincethe4O'st0trytoformabright,uniform,highcorrosi0nandwearresistantco1ourfilmonastainlesssteesurface.Duringtheeariystage,anirnmersionedhodwasused,i.e.inimrs-ingthestainlesssteelinahots0luti…  相似文献   

蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析   总被引：4，自引：0，他引：4  

孟学平高如承  董志国阎斌伦程汉良  李艳杰陈建安 《广东海洋大学学报》2006,26(4):8-13

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。  相似文献   

Bond rolling resistance and its effect on yielding of bonded granulates by DEM analyses     

M. J. Jiang  H. S. Yu  D. Harris 《国际地质力学数值与分析法杂志》2006,30(8):723-761

A discrete element modelling of bonded granulates and investigation on the bond effect on their behaviour are very important to geomechanics. This paper presents a two‐dimensional (2‐D) discrete element theory for bonded granulates with bond rolling resistance and provides a numerical investigation into the effect of bond rolling resistance on the yielding of bonded granulates. The model consists of mechanical contact models and equations governing the motion of bonded particles. The key point of the theory is that the assumption in the original bond contact model previously proposed by the authors (55th CSCE‐ASCE Conference, Hamilton, Ont., Canada, 2002; 313–320; J. Eng. Mech. (ASCE) 2005; 131 (11):1209–1213) that bonded particles are in contact at discrete points, is here replaced by a more reliable assumption that bonded particles are in contact over a width. By making the idealization that the bond contact width is continuously distributed with the normal/tangential basic elements (BE) (each BE is composed of spring, dashpot, bond, slider or divider), we establish a bond rolling contact model together with bond normal/tangential contact models, and also relate the governing equations to local equilibrium. Only one physical parameter β needs to be introduced in the theory in comparison to the original bond discrete element model. The model has been implemented into a 2‐D distinct element method code, NS2D. Using the NS2D, a total of 86 1‐D, constant stress ratio, and biaxial compressions tests have been carried out on the bonded granular samples of different densities, bonding strengths and rolling resistances. The numerical results show that: (i) the new theory predicts a larger internal friction angle, a larger yielding stress, more brittle behaviour and larger final broken contact ratio than the original bond model; (ii) the yielding stress increases nonlinearly with the increasing value of β, and (iii) the first‐yield curve (initiation of bond breakage), which define a zone of none bond breakage and which shape and size are affected by the material density, is amplified by the bond rolling resistance in analogous to that predicted by the original bond model. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

紫阳滑坡治理工程实例分析     

刘满全 《地球科学与环境学报》1994,(2)

本文介绍紫阳骨坡治理工程中有关抗滑桩的设计与施工。文中详细地论述了设计中设计人员应根据工程的具体情况去灵活处理的一些技术问题，如确定有关参数的选择原则，计算上的条件简化和一些具体技术细节的处理方法等．  相似文献   

维生素C对胡子鲶生长、免疫与抗病作用的影响   总被引：2，自引：0，他引：2  

李桂峰  钱沛锋 《广东海洋大学学报》2004,24(4):17-22

在胡子鲶基础料中分别添加 0、5 0 0、10 0 0、15 0 0、2 0 0 0mg/kg的维生素C(VC) ,分别在饲喂不同实验料后的第 12、2 4、36和 5 2天 ,测定各组的生长指标、血清的溶血活力和杀菌活力 ;并在饲喂后的第 4 0天 ,用鲁氏不动杆菌进行攻毒实验 ,观察胡子鲶死亡情况。结果表明 ,添加VC对胡子鲶体重、特定生长率和体长增长率有提高作用 ,但无规律性 ;在添加后的第 12、2 4、36天对血清溶血活力有明显的提高作用 ;对血清杀菌活力没有增强作用。攻毒结果表明 ,VC能明显提高胡子鲶对鲁氏不动杆菌的抵抗力  相似文献   

A simple mathematical model for soil nail and soil interaction analysis   总被引：1，自引：0，他引：1  

Wan-Huan Zhou  Jian-Hua Yin   《Computers and Geotechnics》2008,35(3):479-488

Soil nails have been widely used to stabilize slopes and earth retaining structures in many countries and regions, especially, in Hong Kong. The analysis of the interaction between a soil nail and the surrounding soil is of great interests to both design engineers and researchers. In this paper, authors present a simple mathematical model for the interaction analysis of a soil nail and the surrounding soil considering a few key factors which are soil dilation, bending of the soil nail, vertical pressure, and non-linear subgrade reaction stiffness. The lateral subgrade reaction between the soil and the soil nail is assumed to obey a hyperbolic relation. Reported test data in the literature are used to verify the present model. The contributions of the soil-nail bending on the pull-out resistance are evaluated in two case studies.  相似文献   

鱼生长激素在水产养殖中的应用   总被引：5，自引：0，他引：5  

潘登  双宝  陈荣忠  杨丰 《台湾海峡》2001,20(Z1):85-90

鱼生长激素是鱼类脑垂体中分泌的促进生长的单一亚基的蛋白激素.它参与鱼的生长代谢，能够加速蛋白质合成和脂类降解等生理功能.鱼生长激素能够大大地加快鱼类的生长，在鱼类的水产养殖领域有重大的应用价值，日益引起了人们的关注.现阶段鱼生长激素的应用研究主要集中在转生长激素基因鱼的育种和鱼生长激素的基因工程表达两个方面，且都取得了很大的进展.  相似文献   

盐胁迫对番茄同工酶基因表达的影响     

高明君  常青山  董树刚  刘志鸿  李玉真 《中国海洋大学学报(自然科学版)》1996,(1)

分析了普通番茄（Lycopersicumesculentum）在NaCl胁迫下酯酶（EST）和过氧化物酶（PRX）同工酶的变化，考察了盐胁迫对10个同工酶位点21个等位基因在特定组织中表达的影响。发现许多EST和PRX等位基因在盐胁迫下表达，产生盐诱导同工酶；另有些等位基因的表达受盐抑制，它们在盐胁迫下的表达活性显著减弱或消失。实验表明，EST和PRX同工酶表达的变化与番茄对盐胁迫的遗传适应性有密切关系。  相似文献   

远洋梭子蟹ITS-1基因片段的序列分析     

徐雪娜  吴惠仙  薛俊增  董双林 《海洋学研究》2007,25(4):74-80

采用PCR扩增和序列测定等技术,对远洋梭子蟹Portunus pelagicus核rDNA的第一内转录间隔区(internal transcribed spacer 1,ITS-1)的基因片段进行了初步研究。经PCR扩增和序列测定,得到ITS-1基因片段的碱基序列。该物种ITS-1基因片段的大小为552 bp,碱基A、T、G和C的含量分别为23.00%、25.54%、23.91%和27.54%。同时还对近年来ITS-1在十足目动物分子系统学研究中的应用作一概述并列举了已测出ITS-1序列的十足目动物种类。  相似文献